1. Ensure that the sludge volume of CASS tank is about 30% (that is, the sludge volume of 100ml sewage is about 30ml after 20 minutes of precipitation), stop the water in and out of the anaerobic tank, and add 500kg of aerobic bacteria agent in batches. After adding the bacteria agent, the aeration time of the aerobic area should be maintained at about 20 hours every day.  Every day, 100 kg of flour, 30 kg of urea and 10 kg of phosphate fertilizer are added to CASS pond. If sufficient amount of domestic sewage enters, there is no need to add nutrients.  

2. After 5 days of culture in CASS tank, the activated sludge changed from black to gray in the process of culture, presenting flocculent form (bacteria hanging film).  

3. When the acclimation conditions are available and the effect has been seen in continuous operation, the microorganism can gradually adapt to the new living conditions by increasing the amount of sewage water, and the increase range varies according to the aerobic process and site conditions.  Generally speaking, anaerobic normal start-up can be completed within 4 days, the increase rate is 20%, the index of water intake should not produce too much fluctuation, in this case, the water volume can be gradually increased.